THE ULTIMATE GUIDE TO 2-FDCK HCL

The lab says fixation will trigger the dyes to leak from the cells. I believe the idea is the fact that fixation might destruction cell membranes. I did find a paper which describes fixation employing methanol or acetic acid, just after staining having a DCFDA derivative. DCFDA would be the reagent that stains ROS in the kit ab113851, and I think t